PERTANIKA JOURNAL OF SOCIAL SCIENCES AND HUMANITIES

 

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Home / Regular Issue / JSSH Vol. 32 (1) Jan. 2024 / JST-4197-2023

 

Phenolics-Enhancing Piper sarmentosum (Roxburgh) Extracts Pre-Treated with Supercritical Carbon Dioxide and its Correlation with Cytotoxicity and α-Glucosidase Inhibitory Activities

Mohd Shahrul Ridzuan Hamil, Fauziahanim Zakaria, Lee Chong Yew, Norzilawati Pauzi, Khamsah Suryati Mohd and Noor Hafizoh Saidan

Pertanika Journal of Social Science and Humanities, Volume 32, Issue 1, January 2024

DOI: https://doi.org/10.47836/pjst.32.1.18

Keywords: Cytotoxicity, P. sarmentosum, phenolics, supercritical fluid extraction, α-glucosidase

Published on: 15 January 2024

Piper sarmentosum was found to have various phytochemicals such as polyphenols and flavonoids but also contains asarone isomers, which were reported as carcinogenic in several preclinical studies. Therefore, reducing asarone isomers from this functional food is important while retaining its functional properties. This study compares the total phenolics, total flavonoids, vitexin and naringenin contents, cytotoxicity, and anti-hyperglycaemic activity between various solvent extracts using a two-step extraction with SC-CO2 pre-treatment and a single-step conventional solvent extraction without SC-CO2 pre-treatment. The findings showed that phenolic content was significantly enhanced in ethanol, 50% ethanol, and water extracts pre-treated with SC-CO2, significantly correlated with α-glucosidase inhibitory activity. SC-CO2 pre-treated extracts enhanced the viability of two normal fibroblasts NIH/3T3 and CCD-18Co cell lines. It is concluded that SC-CO2 extraction offers a rapid pre-treatment step to produce safer extracts with better quality and efficacy.

  • Arumugham, T., AlYammahi, J., Rambabu, K., Hassan, S. W., & Banat, S. (2022). Supercritical CO2 pretreatment of date fruit biomass for enhanced recovery of fruit sugars. Sustainable Energy Technologies and Assessments, 52, Article 102231. https://doi.org/10.1016/j.seta.2022.102231

  • Asha, K., Sucheta, G., Kavita, M., Nirmala, D., & Jyoti, S. (2010). Quantification of phenolics and flavonoids by spectrophotometer from Juglans regia. International Journal of Pharma and Bio Sciences, 1(3).

  • Azmi, M. F., Aminuddin, A., Jamal, J. A., Hamid, A. A., & Ugusman, A. (2021). Quantified Piper sarmentosum Roxb. leaves aqueous leaf extract and its antihypertensive effect in dexamethasone-induced hypertensive rats. Sains Malaysiana, 50(1), 171-179. http://dx.doi.org/10.17576/jsm-2021-5001-17

  • Bharathi, A., Roopan, S. M., Vasavi, C. S., Munusami, P., Gayathri, G. A., & Gayathri, M. (2014). In silico molecular docking and in vitro antidiabetic studies of dihydropyrimido [4, 5-a] acridin-2-amines. BioMed Research International, 2014, Article 971569. https://doi.org/10.1155/2014/971569

  • Binh, T. D., Trinh, D. S., & Anna, K. J. (2016). Screening for potential α-glucosidase and α-amylase inhibitory constituents from selected Vietnamese plants used to treat type 2 diabetes. Journal of Ethnopharmacology, 186, 189-195. http://dx.doi.org/10.1016/j.jep.2016.03.060

  • Boik, J. (2001). Natural Compound in Cancer Therapy. Oregon Medical Press.

  • Chen, Y. G., Li, P., Li, P., Yan, R., Zhang, X. Q., Wang, Y., Zhang, X. T., Ye, W. C., & Zhang, Q. W. (2013). α-Glucosidase inhibitory effect and simultaneous quantification of three major flavonoid glycosides in Microctis folium. Molecules, 18(4), 4221-4232. https://doi.org/10.3390/molecules18044221

  • Cheplick, S., Kwon, Y. I., Bhowmik, P., & Shetty, K. (2010). Phenolic-linked variation in strawberry cultivars for potential dietary management of hyperglycemia and related complications of hypertension. Bioresource Technology, 101(1), 404-413. https://doi.org/10.1016/j.biortech.2009.07.068

  • Daud, D., Jalil, U. J. S. A., Sahrol, N. A. A., Zulkefli, S. Z., Adam, N. A., Ishak, N. K., Mahbob, E. N., & Tawang, A. (2021). Comparative anti-obesity and anti-diabetic properties of Piper sarmentosum and Piper betle aqueous extracts via in-vitro system. Journal of Pharmaceutical Advanced Research, 4(11), 1421-1427.

  • Fayaz, S. M., Abdoli, M. A., Baghdadi, M., & Karbasi, A. (2021). Ag removal from e-waste using supercritical fluid: Improving efficiency and selectivity. International Journal of Environmental Studies, 78(3), 459-473. https://doi.org/10.1080/00207233.2020.1834305

  • Feng, J., Yang, X. W., & Wang, R. F. (2011). Bio-assay guided isolation and identification of α-glucosidase inhibitors from the leaves of Aquilaria sinensis. Phytochemistry, 72(2-3), 242-247. https://doi.org/10.1016/j.phytochem.2010.11.025

  • Ghasemzadeh, A., Jaafar, H. Z., & Rahmat, A. (2010). Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules, 15(6), 4324-4333. https://doi.org/10.3390/molecules15064324

  • Hamil, M. S. R., Memon, A. H., Majid, A. M. S. A., & Ismail, Z. (2016). Simultaneous determination of two isomers of asarone in Piper sarmentosum Roxburgh (Piperaceae) extracts using different chromatographic columns. Tropical Journal of Pharmaceutical Research, 15(1), 157-165. https://doi.org/10.4314/tjpr.v15i1.22

  • Hamil, M. S. R., Mohamed, N., & Ismail, Z. (2022). Optimisation of asarone removal from Piper sarmentosum Roxburgh leaves using supercritical carbon dioxide extraction: The Box-Behnken design. Brazilian Journal of Pharmaceutical Sciences, 58, Article e19212. https://doi.org/10.1590/s2175-97902022000X2e19212

  • Hussain, K., Ismail, Z., Sadikun, A., & Ibrahim, P. (2009a). Evaluation of metabolic changes in fruit of Piper sarmentosum in various seasons by metabolomics using Fourier Transform Infrared (FTIR) spectroscopy. International Journal of Pharmaceutical and Clinical Research, 1(2), 68-71.

  • Hussain, K., Ismail, Z., Sadikun, A., Ibrahim, P., & Malik, A. (2009b). Cytotoxicity evaluation and characterization of chloroform extract of leaf of Piper sarmentosum possessing antiangiogenic activity. Pharmacologyonline, 2, 379-391.

  • Hussan, F., Zin, N. N. M., Zulkefli, M. R., Yow, S. C., Abdullah, N. A., & Teoh, S. L. (2013). Piper sarmentosum water extract attenuates diabetic complications in streptozotocin induced sprague-dawley rats. Sains Malaysiana, 42(11), 1605-1612.

  • Ismail, S. M., Hui, C. K., Aminuddin, A., & Ugusman, A. (2018). Piper sarmentosum as an antioxidant: A systematic review. Sains Malaysiana, 47(10), 2359-2368. http://dx.doi.org/10.17576/jsm-2018-4710-12

  • Ko, M. J., Cheigh, C. I., & Chung, M. S. (2014). Relationship analysis between flavonoids structure and subcritical water extraction (SWE). Food Chemistry, 143, 147-155. https://doi.org/10.1016/j.foodchem.2013.07.104

  • Kobori, K., Maruta, Y., Mineo, S., Shigematsu, T., & Hirayama, M. (2013). Polyphenol-retaining decaffeinated cocoa powder obtained by supercritical carbon dioxide extraction and its antioxidant activity. Foods, 2(4), 462-477. https://doi.org/10.3390/foods2040462

  • Krisanapun, C., Wongkrajang, Y., Temsiririrkkul, R., Phornchirasilp, S., & Peungvicha, P. (2012). In vitro evaluation of anti‐diabetic potential of Piper sarmentosum Roxb. extract. The FASEB Journal, 26(S1), 686.7. https://doi.org/10.1096/fasebj.26.1_supplement.686.7

  • Li, P., Liang, Z. H., Jiang, Z., Qiu, Z., Du, B., Liu, Y. B., Li, W. Z., & Tan, L. H. (2018). Supercritical fluid extraction effectively removes phthalate plasticizers in spores of Ganoderma lucidum. Food Science and Biotechnology, 27(6), 1857-1864.

  • Liang, Q., Chow, A. H., Wang, Y., Tong, H. H., & Zheng, Y. (2010). Removal of toxic aristolochic acid components from Aristolochia plants by supercritical fluid extraction. Separation and Purification Technology, 72(3), 269-274. https://doi.org/10.1016/j.seppur.2010.02.016

  • Muhamad, Z., & Mustafa, A. M. (2010). Traditional Malay Medicinal Plants. Penerbit Fajar Bakti Sdn. Bhd.

  • Peungvicha, P., Thirawarapan, S. S., Temsiririrkkul, R., Watanabe, H., Prasain, J. K., & Kadota, S. (1998). Hypoglycemic effect of the water extract of Piper sarmentosum in rats. Journal of Ethnopharmacology, 60(1), 27-32. https://doi.org/10.1016/S0378-8741(97)00127-X

  • Priscilla, D. H., Roy, D., Suresh, A., Kumar, V., & Thirumurugan, K. (2014). Naringenin inhibits α-glucosidase activity: A promising strategy for the regulation of postprandial hyperglycemia in high fat diet fed streptozotocin induced diabetic rats. Chemico-Biological Interactions, 210, 77-85. https://doi.org/10.1016/j.cbi.2013.12.014

  • Rahman, S. F. S. A., Sijam, K., & Omar, D. (2016). Piper sarmentosum Roxb.: A mini review of ethnobotany, phytochemistry and pharmacology. Journal of Analytical & Pharmaceutical Research, 2(5), Article 00031. https://doi.org/10.15406/japlr.2016.02.00031

  • Ridtitid, W., Rattanaprom, W., Thaina, P., Chittrakarn, S., & Sunbhanich, M. (1998). Neuromuscular blocking activity of methanolic extract of Piper sarmentosum leaves in the rat phrenic nerve-hemidiaphragm preparation. Journal of Ethnopharmacology, 61(2), 135-142. https://doi.org/10.1016/S0378-8741(98)00025-7

  • Salehi, P., Asghari, B., Esmaeili, M. A., Dehghan, H., & Ghazi, I. (2013). α-Glucosidase and α-amylase inhibitory effect and antioxidant activity of ten plant extracts traditionally used in Iran for diabetes. Journal of Medicinal Plants Research, 7(6), 257-266. https://doi.org/10.5897/JMPR11.1320

  • Seabra, I. J., Braga, M. E., Batista, M. T., & de Sousa, H. C. (2010). Fractioned high pressure extraction of anthocyanins from elderberry (Sambucus nigra L.) pomace. Food and Bioprocess Technology, 3, 674-683. https://doi.org/10.1007/s11947-008-0134-2

  • Seyyedan, A., Yahya, F., Kamarolzaman, M. F. F., Suhaili, Z., Desa, M. N. M., Khairi, H. M., Somchit, M. N., Fatimah, C. A., Teh, L. K., Salleh, M. Z., & Zakaria, Z. A. (2013). Review on the ethnomedicinal, phytochemical and pharmacological properties of Piper sarmentosum: Scientific justification of its traditional use. CELLMED, 3(3), 19-1. http://dx.doi.org/10.5667/tang.2013.0002

  • Shinde, V., & Mahadik, K. (2019). Supercritical fluid extraction: A new technology to herbals. International Journal of Herbal Medicine, 7(1), 27-34.

  • Subramaniam, V., Adenan, M. I., Ahmad, A. R., & Sahdan, R. (2003). Natural antioxidants: Piper sarmentosum (kadok) and Morinda elliptica (mengkudu). Malaysian Journal of Nutrition, 9(1), 41-51.

  • Sun, X., Chen, W., Dai, W., Xin, H., Rahmand, K., Wang, Y., Zhang, J., Zhang, S., Xu, L., & Han, T. (2020). Piper sarmentosum Roxb.: A review on its botany, traditional uses, phytochemistry, and pharmacological activities. Journal of Ethnopharmacology, 263, Article 112897. https://doi.org/10.1016/j.jep.2020.112897

  • Tajudin, T. J. S. A., Mat, N., Siti-Aishah, A. B., Yusran, A. A. M., Alwi, A., & Ali, A. M. (2012). Cytotoxicity, antiproliferative effects, and apoptosis induction of methanolic extract of Cynometra cauliflora Linn. whole fruit on human promyelocytic leukemia HL-60 cells. Evidence-Based Complementary and Alternative Medicine, 2012, Article 127373. https://doi.org/10.1155/2012/127373

  • Ugusman, A., Zakaria, Z., Hui, C. K., Nordin, N. A. M. M., & Mahdy, Z. A. (2012). Flavonoids of Piper sarmentosum and its cytoprotective effects against oxidative stress. EXCLI Journal, 11, 705-714.

  • Vatai, T., Škerget, M., & Knez, Ž. (2009). Extraction of phenolic compounds from elder berry and different grape marc varieties using organic solvents and/or supercritical carbon dioxide. Journal of Food Engineering, 90(2), 246-254. https://doi.org/10.1016/j.jfoodeng.2008.06.028

  • Vuong, Q. V., & Roach, P. D. (2014). Caffeine in green tea: Its removal and isolation. Separation & Purification Reviews, 43(2), 155-174. https://doi.org/10.1080/15422119.2013.771127

  • Wang, H., Du, Y. J., & Song, H. C. (2010). α-Glucosidase and α-amylase inhibitory activities of guava leaves. Food Chemistry, 123(1), 6-13. https://doi.org/10.1016/j.foodchem.2010.03.088

  • Wongsa, P., Chaiwarit, J., & Zamaludien, A. (2012). In vitro screening of phenolic compounds, potential inhibition against α-amylase and α-glucosidase of culinary herbs in Thailand. Food Chemistry, 131(3), 964-971. https://doi.org/10.1016/j.foodchem.2011.09.088

  • Zhao, J., Yang, J., & Xie, Y. (2019). Improvement strategies for the oral bioavailability of poorly water-soluble flavonoids: An overview. International Journal of Pharmaceutics, 570, Article 118642. https://doi.org/10.1016/j.ijpharm.2019.118642