In general, during the British colonial period of 1910-1957, the agricultural economy of the Johore Malays consisted of a group of farmers who lived in certain settlement areas, especially in the villages. Their agricultural activities can be defined as a group of people who owned or rented lands in small scale and used the manual system in the implementation of their work. This farming community in Johore was usually made up of the Malays. The agricultural activities in Johore in the early 20th century had played an important role to the economic development in the state. Hence, the purpose of this paper is to discuss the patterns of land tenure system that were practised in Johore during the British colonial period from 1910 to 1957. The system had manifested a form of more complex agricultural activity. In fact, land administration during the colonial period had made 'land' as a commercial asset that could be traded. This situation brought a huge impact to the Malays in Johore because it was different from the traditional system which had been practised for centuries. However, the Malays in Johore still enjoyed certain advantages because they still owned their lands until the 'Malay Reservation Enactment' was gazetted in 1913, which brought great influence to the development of agricultural economy of the Malay community. This action was very important in protecting the rights and position of the Johore Malays as the native community in the state. Generally, lands owned privately by the Malays were in small scale. Thus, the situation indirectly led them to undertake agricultural activities which emphasized food crops. This can be seen clearly in several districts in Johore, such as Batu Pahat, Muar, Segamat and Endau. In order to examine the colonial's land administrative system and the impact towards the Malays' agricultural activities in Johore, this study will use primary and secondary sources obtained from the State Archives of Johore. It is hoped that this study can reveal another side of colonialism, the colonial society and socio-economic development in Johore.