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Methylmercury Detection in Maternal Blood Samples by Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry

Amirah Abedinlah, Saliza Mohd Elias, Sarva Mangala Praveena, Suhaili Abu Bakar, Zulida Rejali and Juliana Jalaludin

Pertanika Journal of Social Science and Humanities, Volume 29, Issue 3, July 2021

DOI: https://doi.org/10.47836/pjst.29.3.45

Keywords: LC-ICPMS, maternal blood, methylmercury determination, validation

Published on: 31 July 2021

Methylmercury (MeHg) is one type of mercury (Hg) species known to be very toxic to humans, especially pregnant women and their fetuses. This study aims to obtain and validate the optimum condition of liquid chromatography with inductively coupled plasma mass spectrometry (LC-ICP-MS) to test MeHg concentration. To date, there is limited research that is focused on the maternal blood MeHg samples using LC-ICP-MS in Malaysia. Before analysis, collected blood (500 µL) was placed into a 15 mL polypropylene test tube, followed by the addition of extractant solution [0.10% (v/v) HCl + 0.05% (m/v) L-cysteine + 0.10% (v/v) 2-mercaptoethanol] to the sample and sonicated for 15 minutes. The MeHg level was detected from the sample solution using the LC with Zorbax Eclipse XDB-C18 (4.6 x 12.5 mm, 5 µm) (Agilent Technologies) guard column and analytical column (4.6 x 150 mm, 5 µm) and was quantified by using the ICP-MS. The recovery of MeHg was in the range of 106 to 112% with RSD of less than 10%, followed by the LOD and LOQ values of 0.216 and 0.766 µg/L, respectively. The MeHg appeared at a retention time of fewer than 5 minutes. The results reported that the median (IQR) of maternal blood MeHg level in Malaysian pregnant women was 1.70 (8.90) µg/L, which is 9.7% lower than the LOD value and 11.2% higher than the guideline value of 3.5 µg/L of MeHg in maternal blood.

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ISSN 0128-7702

e-ISSN 2231-8534

Article ID

JST-2500-2021

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