Tritrophic interaction among plants, herbivores and natural enemies of the herbivores is mediated by volatiles. Tritrophic interaction model using rice plant, brown planthopper (BPH), and egg parasitoid of BPH were used to study the volatiles involved in the interaction. Extraction of chemicals in rice plants, determination of the extracted volatiles using GC-MS, and bioassays of the volatiles to analyse the orientation behavior of the egg parasitoid were methods used to study the involvement of the volatiles in the interaction. The knowledge of chemistry involved in such complicated natural interaction is beneficial for enhancing conservation biological control (CBC) of BPH using natural population of its egg parasitoid. Implementation of the positive volatile compounds attract the egg parasitoid as a component of CBC, materials involved that adsorb and release the volatiles efficiently. A material made of integrated bio-silica-cellulose was proven to be an effective adsorbent. Analytical chemistry was important in supporting these findings. Furthermore, in order to increase the efficiency of the volatiles that are positively involved in the tritrophic interaction, developing an attractant formula using synthetic-similar compounds and bio-silica-cellulose adsorbent is valuable in enhancing CBC.