The purpose of this study is to evaluate the ball-milling process for the production of carbon particles from rice straw waste. In the experimental method, carbon particles are prepared by adding heat treatment to the rice straw waste at the temperature of 250°C. The heated rice straw waste is grinded using a conventional grinding method and put into the ball-milling process. Physicochemical properties of carbon particles are evaluated before and after the ball-milling process. The results indicate that sizes of carbon particles decrease significantly from micro (after the conventional grinding process) to submicron range (after additional ball milling process). Almost no changes in the chemical properties of carbon particles are observed. This result implies that the ball-milling process is effective to decrease the size of the prepared carbon particles. The collision phenomenon combined with shear stress among the stainless steel balls is the main reason for breaking carbon particles, making the particle sizes smaller.