The local agricultural industry has produced a lot of biomass waste from agro-based materials, which contain much lignocellulose that can be used as substrates for oyster mushrooms (Pleurotus ostreatus) cultivation. This study aims to compare the effect of different lignocellulose biomass-based substrates on oyster mushrooms’ growth, yield, and nutritional composition. Three different substrates (cassava peel, sugarcane bagasse, and sawdust) were prepared, and the growth response was observed and measured. The fastest mycelial colonization was achieved from sugarcane bagasse after 29 days of cultivation. Oyster mushrooms grown with sugarcane bagasse substrate showed the highest carbohydrate, fiber, and energy content with 10.70%, 7.70%, and 52.00 kcal, respectively, compared to mushrooms grown with other substrates. The biological efficiency of sugarcane bagasse is also comparable to sawdust. Thus, it can be concluded that sugarcane bagasse has a high potential to be used as an alternative biomass-based substrate for cultivating P. ostreatus with enhancement on the growth, yield, and nutritional composition.

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