The objective of this research is to investigate the regulation of apoptotic associated-genes and proteins expression of aloe emodin on oestrogen receptor (ER)-positive (MCF-7). Oestrogen receptor (ER)-positive (MCF-7) cells were cultured in complete RPMI media. Cells were treated with aloe emodin at its IC50 of 80uM. Maximum treatment time was set for 72 hours in all assays. Both genes and proteins involved in the regulation of apoptosis (Fas, FADD, Caspase-3, Caspase-8, Caspase-9, Bax, Bcl-2, and Cytochrome c) in aloe emodin-treated MCF-7 were determined using Quantigene 2.0 Plex and protein ELISA assays respectively. Aloe emodin, previously reported as anti-cancer agent, was found to act as an apoptotic inducer on MCF-7 cells. In intrinsic apoptosis signalling, Bax, Cytochrome c and Caspase-9 proteins were upregulated (54.11% ± 4.51, 25.17% ± 4.13 and 36.05% ±11.75); while no change was observed in Bcl-2 protein. Except for Caspase-9, these results are in accordance with gene expression. In extrinsic apoptosis, Fas and Caspase-8 were upregulated (133.82% ± 2.85 and 26.44% ± 2.48), contrary to gene expression. These findings indicate that aloe emodin activates both extrinsic and intrinsic apoptosis pathways. The data suggests (i) aloe emodin has the potential to be a selective apoptotic inducer in ER+-breast cancer management; and (ii) the present study could be used as a basis for in vivo experiment..