Isozyme variation of 25 isolates of Colletotrichum gloeosporioides from 12 different Malaysian host plants were studied using polyacrylamide gel electrophoresis. The hosts included seven species of legumes and one species each of citrus, grass, cocoa, pepper and chilli. One to five electromorphs per enzyme were produced for the five enzyme systems typed. Some isolates did not produce any bands for the enzymes studied. Double to multiple bands were observed with esterases, MDH, GLDH and LDH. Only one pepper isolate produced a single band for GDH. Some variations among the isolates of different host plants were observed with the isozyme patterns of esterases and MDH. Isozyme tests may prove to be a useful tool in the identification of C. gloeosporioides of different host plants when more samples are tested in future.