Low number of fruit production becomes the main constraint to develop Jatropha curcas as one of the alternative biodiesel sources in Malaysia. Therefore, one study was carried out to evaluate the effects of ethephon by combining it with urea for induction of flowering in Jatropha, with the main objective of increasing the productivity of fruit. The experiment was carried out at Rubber Research Institute Experimental Station (RRIES) Sungai Buloh, Selangor, where the soil was identified as the Renggam series. The planting material used was Jatropha curcus from Thailand accession. There were a total of nine treatments which were arranged according to Randomized Complete Design (RCD). Results indicated that Treatment 2 (200ppm ethephon 2.5% + 2% urea) showed the best combination promote in balancing male-female ratio, and resulted in an improved yield of the plants expressed as an increase in fruit produce and ripe fruit. The efficacy of ethephon at a lower concentration for increased fruit yield, with the addition of urea, is of great deal as this will lead to decreased costs and increased survival of Jatropha plantation.