Standardized and optimized methods in preservation and storage of RNA samples on three different tissues were elucidated on fish tissues. The treatments used included excision of fresh tissues, preservation of tissues by immersing completely in RNAlater followed by immediate freezing at -80°C prior to RNA assays. Current data suggests that preserving fish tissues in RNAlater with subsequent storage at -80°C produced satisfactory amount of RNA from dorsal fin and gonad, but not from muscle tissues. The optimized method will provide an alternative storage method option and enables greater usage of RNAlater for preserving RNA from animal cells.