Glomus fasciculatum pot cultures were raised in Rhodes grass (Chloris gayana Kunth) using perlite - soilrite mix (1:1 by volume) in 16 cm pots holding 700 g substrate and were harvested at different intervals. Cultures harvested on the 75th day after sowing had the maximum number of infective propagules. In a second experiment, pruning was found tc reduce the mycorrhizal propagules up to 4 weieks. However, the propagules increased from 5th week onwards