PERTANIKA JOURNAL OF SCIENCE AND TECHNOLOGY

 

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Effects of Various Doses of Saccharomyces cerevisiae on the Growth, Survival Rate, and Blood Profile of Saline Red Tilapia (Oreochromis spp.) in the Semi-Intensive Culture Conditions

Istiyanto Samidjan, Diana Rachmawati, Safar Dody and Putut Har Riyadi

Pertanika Journal of Science & Technology, Volume 31, Issue 1, January 2023

DOI: https://doi.org/10.47836/pjst.31.1.31

Keywords: Immune system, Saccharomyces cerevisiae, saline red tilapia, semi-intensive intensive

Published on: 3 January 2023

The availability of good feeding on fish performance is needed to improve the poor aquaculture condition. The study examines the effects of Saccharomyces cerevisiae supplementation on the growth, survival rate, and blood profile of saline red tilapia in the semi-intensive culture. Sixty saline red tilapia (4.58 ± 0.09 g) were divided into five groups and received standard feed with supplementation of S. cerevisiae at different doses: (A-E) 0, 5, 10, 15, and 20 g/kg feed. S. cerevisiae was sprayed onto the feed and given to fish three times/day for 49 days. The efficiency of feed utilization (EFU), feed conversion ratio (FCR), the protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and blood profiles were examined. The water quality parameters were also evaluated, including temperature, pH, dissolved oxygen, NH3, and salinity. Data were analyzed using ANOVA followed by the Duncan test. The supplementation of 10 g/kg S. cerevisiae could significantly (p<0.05) increase EFU (85.13%), PER (3.76), RGR (4.53%), and survival rate (97.53%), and decrease FCR (1.42) of the saline red tilapia. All doses of S. cerevisiae significantly (p<0.05) increased the number of hematocytes (34.68, 35.96, 34.14, and 34.52%, respectively) and hemoglobin (12.98, 13.58, 12.73, and 12.67%, respectively) of fish. The highest number of erythrocytes and leucocytes was found in dose 10 g/kg feed. All water parameters were stable during the study. Therefore, S. cerevisiae can be used as a feed supplement for red tilapia fish to increase the growth, survival rate, and blood profile of the saline red tilapia.

  • Abdel-Tawwab, M., Mousa, M. A. A., & Mohammed, M. A. (2010). Use of live baker’s yeast, Saccharomyces cerevisiae, in practical diet to enhance the growth performance of galilee tilapia, Sarotherodon galilaeus (L.), and its resistance to environmental copper toxicity. Journal of the World Aquaculture Society, 41(S2), 214-223. https://doi.org/10.1111/j.1749-7345.2010.00361.x

  • Abu-Elala, N., Marzouk, M., & Moustafa, M. (). Use of different Saccharomyces cerevisiae biotic forms as immune-modulator and growth promoter for Oreochromis niloticus challenged with some fish pathogens. International Journal of Veterinary Science and Medicine, 1(1), 21-29. https://doi.org/10.1016/j.ijvsm.2013.05.001

  • Agboola, J. O., Overland, M., Skrede, A., & Hansen, J. O. (2021). Yeast as major protein-rich ingredient in aquafeeds: A review of the implications for aquaculture production. Reviews in Aquaculture, 13(2), 949-970. https://doi.org/10.1111/raq.12507

  • AOAC. (2019). Official methods of analyses (21st Ed.). Association of Official Analytical Chemists Inc https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/

  • APHA (2017). Standard methods for the examination of water and wastewater (23rd Ed.). American Water Works Association. https://beta-static.fishersci.com/content/dam/fishersci/en_US/documents/programs/scientific/technical-documents/white-papers/apha-water-testing-standard-methods-introduction-white-paper.pdf

  • Ayuniar, L. N., & Hidayat, J. W. (2018). The profile quality of pond In Kendal Regency to diversification aquaculture. Proceedings of E3S Web of Conferences, 31(08025), 1-5. https://doi.org/10.1051/e3sconf/20183108025

  • de Azevedo, R. V., Filho, J. C. F., Pereira, S. L., Cardoso, L. D., Andrade, D. R., & Júnior, M. V. (2016). Dietary mannan oligosaccharide and Bacillus subtilis in diets for Nile tilapia (Oreochromis niloticus). Acta Scietarium, 38(4), 347-353. http://dx.doi.org/10.4025/actascianimsci.v38i4.31360

  • Goda, A. M. A., Mabrouk, H. A. H. H., Wafa, M. A. E., & El-Afifi, T. M. (2012). Effect of using baker’s yeast and exogenous digestive enzymes as growth promoters on growth, feed utilization and hematological indices of Nile Tilapia, Oreochromis niloticus fingerling. Journal of Agricultural Sciene and Technology, B(2), 15-28.

  • Huyben, D., Nyman, A., Vidakovic, A., Passoth, V., Moccia, R., Kiessling, A., Dicksved, J., & Lundh, T. (2017). Effect of dietary inclusion of the yeasts Saccharomyces cerevisiae and Wickerhamomyces anomalus on gut microbiota of rainbow trout. Aquaculture, 473, 528-537. http://dx.doi.org/10.1016/j.aquaculture.2017.03.024

  • Manoppo, H., & Kolopita, M. E. F. (2015). Pengimbuhan ragi roti dalam pakan meningkatkan respons imun nonspesifik dan pertumbuhan ikan Nila [Supplementation of baker’s yeast in feed enhance nonspecific immune response and growth of Nile tilapia]. Jurnal Veteriner, 16(2), 204-211.

  • Manoppo, H., & Kolopita, M. E. F. (2016). Use of bread yeast (Saccharomyces cerevisiae) as immunostimulant to increase resistance of carp (Cyprinus carpio L) against Aeromonas hydrophila bacterial infection. Jurnal Budidaya Perairan, 4(3), 37-47. https://doi.org/10.35800/bdp.4.3.2016.14945

  • Mohammadi, F., Mousavi, S. M., Zakeri, M., & Ahmadmoradi, E. (2016). Effect of dietary probiotic, Saccharomyces cerevisiae on growth performance, survival rate and body biochemical composition of three spot cichlid (Cichlasoma trimaculatum). AACL Bioflux, 9(3), 451-457.

  • Mohammed, H., Olivares-Fuster, O., Lafrentz, S., & Arias, C. R. (2013). New attenuated vaccine against columnaris disease in fish: Choosing the right parental strain is critical for vaccine efficacy. Vaccine, 31(45), 5276-80. https://doi.org/10.1016/j.vaccine.2013.08.052

  • Rachmawati, D., Istiyanto, S., & Elfitasari, T. (2018). Effect of the phytase enzyme addition in the artificial feed on digestibility of feed, feed conversion ratio and growth of gift tilapia saline fish (Oreochromis niloticus) nursery stadia I. IOP Conference Series Earth and Environmental Science, 116(012009), 1-12. http://dx.doi.org/10.1088/1755-1315/116/1/012009

  • Rachmawati, D., Istiyanto, S., & Mel, M. (2017). Effect of phytase on growth performance, feed utilization efficiency and nutrient digestibility in fingerlings of Chanos chanos (Forsskal 1775). Philippine Journal of Science, 146(3), 237-245.

  • Rachmawati, D., Istiyanto, S., Ristiawan A. N, & Susilowati, T. (2019a). Effects of Sacharomyces cereviceae incorporated diet on growth performance, apparent digestibility coefficient of protein and survival rate of catfish (Pangasius hypothalamus). Aquacultura Indonesiana, 20(1), 8-14. http://dx.doi.org/10.21534/ai.v20i1.136

  • Rachmawati, D., Johannes, H., Istiyanto, S., Vivi, E. H., & Windarto, S. (2019b). The effects of Saccharomyces cerevisiae-enriched diet on feed usage efficiency, growth performance and survival rate in Java barb (Barbonymus gonionotus). AACL Bioflux, 12(5), 1841-1849.

  • Rajagukguk, B. B., Lumenta, C., & Mokolensang, J. F. (2017). Pemanfaatan ragi (Saccharomyces cerevisiae) pada formulasi pakan dalam meningkatkan pertumbuhan ikan Nila (Oreochromis niloticus) [Utilization of yeast (Saccharomyces cerevisiae) in feed formulations in increasing the growth of tilapia (Oreochromis niloticus]. Jurnal Budidaya Perairan, 5(3), 44-49. https://doi.org/10.35800/bdp.5.3.2017.17887

  • Rawung, M. E., & Manoppo, H. (2014). Penggunaan ragi roti (Saccharomyces cereviciae) secara in situ untuk meningkatkan respon kebal non-spesifik ikan nila (Oreochromis niloticus) [In situ use of baker’s yeast (Saccharomyces cereviciae) to enhance the non-specific immune response of tilapia (Oreochromis niloticus)]. Jurnal Perikanan and Kelautan, 2(2), 7-14. https://doi.org/10.35800/bdp.2.2.2014.4901

  • Razak, A. P., Kreckhoff, R. L., & Watung, J. C. (2017). Administrasi oral imunostimulan ragi roti (Saccharomyces cerevisiae) untuk meningkatkan pertumbuhan ikan mas (Cyprinus carpio L.) [Oral administration of Saccharomyces cerevisiae as immunostimulant to increase growth of carp, Cyprinus Carpio L)]. Jurnal Budidaya Perairan, 5(2), 27-36. https://doi.org/10.35800/bdp.5.2.2017.16637

  • Rodrigues, M. V., Zanuzzo, F. S., Koch, J., de Oliveira, C., Sima, P., & Vetvicka, V. (2020). Development of fish immunity and the role of β-Glucan in immune responses. Molecules, 25(22), Article 5378. https://doi.org/10.3390/molecules25225378

  • Sheikhzadeh, A. E., Heidarich, M., Pashaki, A. K., Nofouzi, K., Farshabi, M. A., & Akbari, M. (2012). Hilyses®, fermented Saccharomyces cerevisiae, enhances the growth performance and skin non-specific immune parameters in rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology, 32(6), 1083-1087. https://doi.org/10.1016/j.fsi.2012.03.003

  • Sitohang, R. V., Herawati, T., & Lili, W. (2012). Pengaruh pemberian dedak hasil fermentasi ragi (Saccharomyces cereviciae) terhadap pertumbuhan biomass Daphnia sp [The Effect of fermented yeast bran (Saccharomyces cereviciae) on biomass growth of Daphnia sp.]. Jurnal Perikanan and Kelautan, 3(1), 65-72.

  • Statistics of Jawa Tengah Province. (2018). Production and production value of aquaculture by regency/municipality and main commodity in Jawa Tengah Province, 2018. https://jateng.bps.go.id/statictable/2020/07/22/1953/produksi-dan-nilai-produksi-perikanan-budidaya-menurut-kabupaten-kota-dan-komoditas-utama-di-provinsi-jawa-tengah-2018x.html

  • Steel, R. G. D., Torrie, J. H., & Dickey, D. A. (1996). Principles and procedures of statistics (3rd Ed.). McGraw Hill International Book Company, Inc.

  • Tewary, A., & Patra, B. C. (2011). Oral administration of baker’s yeast (Saccharomyces cerevisiae) acts as a growth promoter and immunomodulator in Labeo rohita (Ham.). Journal of Aquaculture Research & Development, 2(1), 1-7. https://doi.org/10.4172/2155-9546.1000109

  • Tovar, D., Zambonino, J., Cahu, C., Gatesoupe, F. J., Va´zquez-Ju´arez, R., & Le´sel, R. (2002). Effect of live yeast incorporation in compound diet on digestive enzyme activity in sea bass (Dicentrarchus labrax) larvae. Aquaculture, 204(1-2), 113-123. https://doi.org/10.1016/S0044-8486(01)00650-0

  • Welker, T. L., Lim, C., Yildirim-Aksoy, M., Shelby, R., & Klesius, P. H. (2007). Immune response and resistance to stress and Edwardsiella ictaluri challenge in channel catfish, Ictalurus punctatus, fed diets containing commercial whole-cell yeast or yeast subcompo-nents. Journal of the World Aquaculture Society, 38(1), 24-35. https://doi.org/10.1111/j.1749-7345.2006.00070.x

ISSN 0128-7680

e-ISSN 2231-8526

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JST-3469-2022

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