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Physicochemical Characterisation and Substrate Specificity of Purified ß-1,6-glucanase from Trichoderma longibrachiatum

Muskhazli Mustafa, Nor Azwady Abd. Aziz, Anida Kaimi, Nurul Shafiza Noor, Salifah Hasanah Ahmad Bedawi and Nalisha Ithnin

Pertanika Journal of Tropical Agricultural Science, Volume 17, Issue 1, January 2009

Keywords: ß-1,6-glucanase, characterisation, metal ion, pH, purification, substrate specificity, Trichoderma longibrachiatum

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The ß-1,6-glucanases are ubiquitous enzymes which appear to be implicated in the morphogenesis and have the ability to become virulence factor in plant-fungal symbiotic interaction. To our knowledge, no report on ß-1,6-glucanases purification from Trichoderma longibrachiatum has been made, although it has been proven to have a significant effect as a biocontrol agent for several diseases. Therefore, the aim of this study was to purify ß-1,6- glucanase from T. longibrachiatum T28, with an assessment on the physicochemical properties and substrate specificity. ß-1,3-glucanase enzyme, from the culture filtrate of T. longibrachiatum T28, was successively purified through precipitation with 80% acetone, followed by anionexchange chromatography on Neobar AQ and chromatofocusing on a Mono P HR 5/20 column. (One ß-1,6-glucanase) band at 42kDa in size was purified, as shown by the SDS-PAGE. The physicochemical evaluation showed an optimum pH of 5 and optimum temperature of 50°C for enzyme activity with an ability to maintain 100% enzyme stability. Enzyme activity was slightly reduced by 10-20% in the presence of 20 mM of Zn2+, Ca2+, Co2+, Mg2+, Cu2+, Mn2+ and Fe2+. The highest ß-1,6-glucanase hydrolysis activity was obtained on pustulan due to the similarity of ß-glucosidic bonds followed by laminarin, glucan and cellulose. Therefore, it can be concluded that the characterization of ß-1,6- lucanase secreted by T. longibrachiatum in term of molecular weight, responsed to selected physicochemical factors and the substrate specificity are approximately identical to other Trichoderma sp.

ISSN 1511-3701

e-ISSN 2231-8542

Article ID

JST-0077-2008

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