The production of ethanol, from glucose in batch and fed batch culture, was investigated. In the fed batch culture, the glucose feeding was added into the culture at 16^th hour of fermentation. The effects of different glucose concentration feeding rates on ethanol fermentation were investigated for fed batch culture. The 2gL^-1hr^-1 glucose concentration feeding rate was found to give higher ethanol yield (2.47 g ethanol g glucose^-1), with respect to substrate consumed as compared to 8 gL^-1hr^-1 (0.23 g ethanol g glucose^-1) and 4 gL^-1hr^-1 (0.20 g ethanol g glucose^-1). The ethanol yield with respect to substrate consumed obtained in batch culture was 0.81 g ethanol g glucose^-1. The fed batch culture at 2 gL^-1hr^-1 glucose concentration feeding rate was proven to be a better fermentation system than the batch culture. The specific growth rate, specific glucose consumption rate and specific ethanol production rate for the fed batch fermentation, at 2 gL^-1hr^-1 glucose concentration feeding rate, were 0.065 hr^-1, 1.20 hr^-1 and 0.0009 hr^-1, respectively.