A study was carried out to evaluate the efficacy of Trichoderma-induced suppressive soil on growth of tomato cv Baccarat 322 and on wilt disease caused by Fusarium oxysporum f. sp. lycopersici (Fol). Dry preparation of T. virens (UPM 23), T. harzianum (UPM 40), singly and as mixtures (UPM 2340) with organic compost as carrier were amended into soil mixture as treatments to induce disease suppressiveness. The wilt fungus at the inoculum level of 100 mL plant (9 x 10⁶ spore mL^-1) caused significant suppression in growth and yield. However, the Trichoderma-induced suppressive soil checked the suppressive effect of the fungus leading to significant increase in root dry weight and yield compared with the inoculated control. Disease incidence expressed as the area under the disease progress curve (AVDPC) was highest for the control plants and lowest for plants treated with UPM 2340 followed by UPM 40 and UPM 23. The disease progress rate was significantly lower in plants treated with UPM 2340 (rm = 0.01) compared to non-treated plants (rm = 0.75). Rhizosphere population of the introduced Trichoderma (colony forming units g¹ soil) gradually decreased with an increase in frequency recovered from the tomato roots, suggesting an ability of the Trichoderma to colonize the roots.