Colletotrichum gloeosporioides from eighteen Malaysian hosts, namely mandarin orange (Citrus reticulata), cacao (Theobroma cacao), an orchid (Cattleya sp.), pepper (Piper nigrum ), chilli (Capsicum annum ), grass (Imperata cylindrica ), mango (Mangifera indica) and legume cover crops (P u e ra ria phaeseoloides, Centrosema pubescens, and Calopogonium mucunoides) and a weed (Mimosa pudica), vegetables (Psophocarpus tetragonolobus, Phaseolus vulgaris, Vigna radiata , Vigna sesquipedalis and Arachis hypogaea), a shrub (Leucaena leucocephala) and a herbaceous vine (Clitoria ternatea ) were examined fo r colony growth characteristics, morphology o f conidia, growth on various media and temperatures and pathogenicity on Phaeseolus bean hypocotyls. Conidium size o f Colletotrichum gloeosporioides was 14.25-19.0 x 2.7-5.03 fim. The appressorium size was 6.34-10.08 x 5.28-7.31 fim and the shape was globose/sub-globose/lobed. No correlation between the appressorium size and shape was noticed. The optimum temperature for growth was 28 and 30°C. None o f the isolates caused infection symptoms on Phaseolus bean hypocotyls.