PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

Liberica coffee is a minor species that is planted all around the world. Therefore, there is little study conducted on this coffee species as only one percent is cultivated all around the world. In Malaysia, there is still no research focusing on coffee pulp from Sarawak liberica sp. and thus leading to this study. The wastes and by-product such as coffee pulps will become the residues as they were not needed in processing the coffee. This will create environmental pollution. Thus, this research aimed to evaluate the feasibility study on the physicochemical properties of coffee pulp from Sarawak liberica sp. including determination by colorimetric assays for phenolic and flavonoid content, antioxidant activity, and reducing sugar analysis. The antibacterial activities of coffee pulp were evaluated against Gram-positive, Staphylococcus aureus, and Gram-negative Salmonella typhimurium using a disc diffusion method. As a result, Sarawak liberica sp. coffee pulp extract contained total phenolic content of 24.24 mg GAE/g of coffee pulp, a total flavonoid content of 39.39 mg QE/g of coffee pulp, DPPH scavenging activity of 92.24 ± 0.03%, reducing sugar analysis of 13.13 mg GE/g of coffee pulp, and there was no significant effect of antibacterial activities. Therefore, the physicochemical study determination in this study would add values toward Sarawak liberica sp. coffee pulp by-product and thus reducing the disposal of Liberica coffee wastes in the future.