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Pertanika Journal of Tropical Agricultural Science

Official journal of Universiti Putra Malaysia for peer-reviewed research across tropical agriculture and allied agricultural sciences.

e-ISSN 2231-8542 ISSN 1511-3701
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Research article

Effects of Coconut Water and Banana Homogenate on Shoot Regeneration of Meyer Lemon (Citrus × meyeri)

Stephanie Qiao Er Wong, Najwa Amalina Haradzi, Dahmendra Sriskanda, Sreeramanan Subramaniam and Bee Lynn Chew

Volume 47, Issue 1 February 2024 Published 23 Febuary 2024
https://doi.org/10.47836/pjtas.47.1.11
KeywordsBanana homogenate, Citrus × meyeri, coconut water, Meyer lemon, organic additive, shoot regeneration
Article content

Abstract

Meyer lemon (Citrus × meyeri) is a hybrid citrus fruit from the Rutaceae family, originating from China. It is well-known for its distinctive appearance and flavor, as well as its health-nourishing nutrients. Micropropagation is an efficient alternative in the multiplication of plant stocks suitable for the commercial scale. The inclusion of organic additives in culture media has been found to provide a cost-effective option as a plant growth stimulant for in vitro plant development. The current study intends to assess the effects of coconut water and banana homogenate in the regeneration of Meyer lemon. In vitro, shoots were treated in half-strength Murashige and Skoog media fortified with 2 mg/L 6-benzylaminopurine with varying concentrations of coconut water and banana homogenate without sucrose. Results revealed that the treatment of 30% coconut water and 40 g/L banana homogenate resulted in the greatest proliferation of new shoots (3.00 ± 0.873 and 1.57 ± 0.297, respectively), whereas treatment of 40% coconut water resulted in the greatest shoot elongation of 0.239 ± 0.026 cm. The current study suggested the incorporation of coconut water and banana homogenate as potential substitutes for carbon sources and growth stimulants in the regeneration of Meyer lemon.
Supporting literature

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