PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE

This research explores the potential of Ajwa date fruit extract as a bioactive component in cosmeceutical formulations, with particular emphasis on its anti-tyrosinase, anti-collagenase, and ultraviolet (UV) protection capabilities. The antioxidant activity was determined using the design of response surface methodology, considering the factor of sample-to-water-as-solvent ratio, extraction time, and size of the sample. This factor possesses the capacity to exert an influence on the production of antioxidants and enhance the efficacy of the extraction procedure. The Ajwa date fruit extract was obtained using the Soxhlet method. The extract showed notable inhibition percentages of 67.77 and 49.12 for anti-tyrosinase and anti-collagenase activities, respectively. Additionally, it revealed a sun protection factor value of 17.09. Previous research has indicated that Ajwa dates exhibit significant inhibitory properties against tyrosinase and collagenase enzymes, making them potentially valuable in cosmetic applications. Therefore, research has demonstrated this study’s promise in skin pigmentation, elasticity, and UV protection. The study places significant importance on exploring natural alternatives in cosmetics. It highlights the encouraging outcomes obtained from using Ajwa date fruit extract, emphasising its potential for future advancements in cosmeceuticals. The present study offers a valuable opportunity to produce skincare formulas that are both safer and more effective.

• Alharbi, K. L., Raman, J., & Shin, H.-J. (2021). Date fruit and seed in nutricosmetics. Cosmetics, 8(3), 59. https://doi.org/10.3390/cosmetics8030059

• Al-Shahib, W., & Marshall, R. J. (2003). The fruit of the date palm: Its possible use as the best food for the future? International Journal of Food Sciences and Nutrition, 54(4), 247–259. https://doi.org/10.1080/09637480120091982

• Bigg, H. F., Clark, I. M., & Cawston, T. E. (1994). Fragments of human fibroblast collagenase: Interaction with metalloproteinase inhibitors and substrates. Biochim Biophys Acta - Protein Structure and Molecular Enzymology, 1208(1), 157-165. https://doi.org/10.1016/0167-4838(94)90173-2

• de Oliveira, B. H., Packer, J. F., Chimelli, M., & de Jesus, D. A. (2007). Enzymatic modification of stevioside by cell-free extract of Gibberella fujikuroi. Journal of Biotechnology, 131(1), 92–96. https://doi.org/10.1016/j.jbiotec.2007.05.035

• Fonseca, A. P., & Rafaela, N. (2013). Determination of sun protection factor by UV-Vis spectrophotometry. Health Care: Current Reviews, 1(1), 1000108. https://doi.org/10.4172/hccr.1000108

• Haliloglu, Y., Ozek, T., Tekin, M., Goger, F., Baser, K. H. C., & Ozek, G. (2017). Phytochemicals, antioxidant, and antityrosinase activities of Achillea sivasica Çelik and Akpulat. International Journal of Food Properties, 20(sup1), S693–S706. https://doi.org/10.1080/10942912.2017.1308954

• Herrling, T., Jung, K., & Fuchs, J. (2006). Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 63(4), 840–845. https://doi.org/10.1016/j.saa.2005.10.013

• Hong, Y. J., Tomas-Barberan, F. A., Kader, A. A., & Mitchell, A. E. (2006). The flavonoid glycosides and procyanidin composition of Deglet Noor dates (Phoenix dactylifera). Journal of Agricultural and Food Chemistry, 54(6), 2405–2411. https://doi.org/10.1021/jf0581776

• Momtaz, S., Lall, N., & Basson, A. (2008). Inhibitory activities of mushroom tyrosine and DOPA oxidation by plant extracts. South African Journal of Botany, 74(4), 577–582. https://doi.org/10.1016/j.sajb.2008.02.005

• Morocho-Jácome, A. L., Freire, T. B., de Oliveira, A. C., de Almeida, T. S., Rosado, C., Velasco, M. V. R., & Baby, A. R. (2021). In vivo SPF from multifunctional sunscreen systems developed with natural compounds — A review. Journal of Cosmetic Dermatology, 20(3), 729–737. https://doi.org/10.1111/jocd.13609

• Petruzzi, D. (2024). Cosmetics industry - Statistics and facts. Statista. https://www.statista.com/topics/3137/cosmetics-industry/#topicOverview

• Ribeiro, A. S., Estanqueiro, M., Oliveira, M. B., & Lobo, J. M. S. (2015). Main benefits and applicability of plant extracts in skin care products. Cosmetics, 2(2), 48–65. https://doi.org/10.3390/cosmetics2020048

• Shin, J.-W., Kwon, S.-H., Choi, J.-Y., Na, J.-I., Huh, C.-H., Choi, H.-R., & Park, K.-C. (2019). Molecular mechanisms of dermal aging and antiaging approaches. International Journal of Molecular Sciences, 20(9), 2126. https://doi.org/10.3390/ijms20092126

• Shin, S., Cho, S. H., Park, D., & Jung, E. (2020). Anti-skin aging properties of protocatechuic acid in vitro and in vivo. Journal of Cosmetic Dermatology, 19(4), 977–984. https://doi.org/10.1111/jocd.13086

• Suva, M. A. (2014). Evaluation of sun protection factor of zingiber officinale roscoe extract by ultraviolet spectroscopy method. Jornal of PharmaSciTech, 3(2), 95-97.

• Utami, S., Sachrowardi, Q. R., Damayanti, N. A., Wardhana, A., Syarif, I., Nafik, S., Arrahmani, B. C., Kusuma, H. S. W., & Widowati, W. (2018). Antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorrhiza Miq.) fruit as antiaging. Journal of Herbmed Pharmacology, 7(2), 88–93. https://doi.org/10.15171/jhp.2018.15

• Vijayakumar, R., Gani, S. S. A., & Mokhtar, N. F. (2017). Anti-elastase, anti-collagenase, and antimicrobial activities of the underutilized red pitaya peel: An in vitro study for anti-aging applications. Asian Journal of Pharmaceutical and Clinical Research, 10(8), 251-255. https://doi.org/10.22159/ajpcr.2017.v10i8.19048

• Vujanović, M., Majkić, T., Zengin, G., Beara, I., Tomović, V., Šojić, B., Đurović, S., & Radojković, M. (2020). Elderberry (Sambucus nigra L.) juice as a novel functional product rich in health-promoting compounds. RSC Advances, 10(73), 44805–44814. https://doi.org/10.1039/d0ra09129d

• Willcox, J. K., Ash, S. L., & Catignani, G. L. (2004). Antioxidants and prevention of chronic disease. Critical Reviews in Food Science and Nutrition, 44(4), 275–295. https://doi.org/10.1080/10408690490468489

• Zduńska, K., Dana, A., Kolodziejczak, A., & Rotsztejn, H. (2018). Antioxidant properties of ferulic acid and its possible application. Skin Pharmacology and Physiology, 31(6), 332–336. https://doi.org/10.1159/000491755

• Zengin, G., Uysal, S., Ceylan, R., & Aktumsek, A. (2015). Phenolic constituent, antioxidative, and tyrosinase inhibitory activity of Ornithogalum narbonense L. from Turkey: A phytochemical study. Industrial Crops and Products, 70, 1–6. https://doi.org/10.1016/j.indcrop.2015.03.012