e-ISSN 2231-8542
ISSN 1511-3701

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Identification and factors affecting in-vitro growth of an indigenous mushroom, Boletussp. from Bachok, Kelantan, Malaysia

Lau Meng Fei, Rosnida Tajuddin, Masratul Hawa Mohd and Latiffah Zakaria

Pertanika Journal of Tropical Agricultural Science, Volume 40, Issue 1, February 2017

Keywords: wild mushroom, Boletus griseipurpureus, in-vitro growth, toxicity, solid media, liquid media

Published on: 21 Feb 2017

Wild mushrooms including Boletus are among edible mushrooms consumed by local populations. However, the species identity for many types of wild edible mushroom in Malaysia is poorly known. The present study was conducted to identify and to determine factors affecting in-vitro growth of an edible Boletussp. (kulat gelam) commonly found in peat swamp forests in Bachok, Kelantan. Identification was initially done using macroscopic and microscopic characteristics of the fruiting bodies. However, due to overlapping chemical colour reactions, morphological and anatomical characteristics of the fruiting body with other Boletus species, species identity was confirmed using ITS region, and the mushroom was identified as Boletus griseipurpureus. A toxicity test indicated that B. griseipurpureus is an edible mushroom with low toxic levels (LC50=4.33 mg/mL). From growth studies, the results suggested that potato dextrose agar (PDA), cassava dextrose agar (CDA) and yeast malt extract (YME) were the most suitable artificial media for mycelial growth of B. griseipurpureus at pH 6.0 and 30°C. To our knowledge, this is the first documented report on wild edible mushroom B. griseipurpureus in Malaysia.

ISSN 1511-3701

e-ISSN 2231-8542

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