Research article
Effect of Various Immersion Time and Water Temperature on Seed Germination of Clitoria ternatea and Momordica charantia
https://doi.org/10.47836/pjtas.44.4.03KeywordsClitoria ternatea, germination, herbal plants, Momordica charantia, seed germination
Article content
Abstract
The seeds of Clitoria ternatea and Momordica charantia were subjected to seven pre-sowing treatments, i.e., control (T0), peeled coat and soaked in 5 oC for 24 hours (T1), peeled coat and soaked in 37 oC for 24 hours (T2), peeled coat and soaked in 5 oC for 48 hours (T3), peeled coat and soaked in 37 oC for 48 hours (T4), peeled coat and soaked in 5 oC for 48 hours (T5), peeled coat and soaked in 37 oC for 72 hours (T6). The study revealed that peeling the coat and soaking seeds in water for various temperatures and periods improved seed germination. The highest germination and germination energy percentage of C. ternatea were observed in T2, namely 94.95% and 23.69%, respectively, while the lowest germination (0%) and germination energy (0%) was found in T6. The highest germination and germination energy percentage of M. charantia, namely 64.38%, and 16.10%, respectively, were found in T3, while the lowest germination (10.67%) and germination energy (2.17%) were observed in T0. The germination may vary for both seeds used in the study as C. ternatea and M. charantia are different in type. The pre-sowing treatments of seeds would prove its potential in the practical fields.
Supporting literature
References
- Alsarhan, A., Sultana, N., Al-Khatib, A., & Kadir, M. R. A. (2014). Review on some Malaysian traditional medicinal plants with therapeutic properties. Journal of Basic and Applied Sciences, 10, 149-159. http://doi.org/10.6000/1927-5129.2014.10.20
- Azad, M. S., Zedan-Al-Musa, M., & Matin, M. A. (2010). Effects of pre-sowing treatments on seed germination of Melia azedarach. Journal of Forestry Research, 21(2), 193-196. http://doi.org/10.1007/s11676-010-0031-1
- Catalan, L. A., & Macchiaveloli, R. (1991). Improving germination in Prosopis flexuosa D. C. and P. alba Griseb. with hot water treatments and scarification. Seed Science and Technology, 19(2), 253-262.
- Corner, E. J. H. (1976). The seeds of dicotyledons (Vol. 1). Cambridge University Press.
- Czabator, F. J. (1962). Germination value: An index combining speed and completeness of pine seed germination. Forest Science, 8(4), 386-396.
- Dove, N. (2010). The effect of increasing temperature on germination of native plant species in the North Woods region. https://underc.nd.edu/assets/156376/fullsize/dove2010.pdf
- Enioutina, E. Y., Salis, E. R., Job, K. M., Gubarev, M. I., Krepkova, L. V., & Sherwin, C. M. (2017). Herbal medicines: Challenges in the modern world. Part 5. Status and current directions of complementary and alternative herbal medicine worldwide. Expert Review of Clinical Pharmacology, 10(3), 327-338. https://doi.org/10.1080/17512433.2017.1268917
- Hossain, M. A., Arefin, M. K., Khan, B. M., & Rahman, M. A. (2005). Effects of seed treatments on germination and seedling growth attributes of Horitaki (Terminalia chebula Retz.) in the nursery. Research Journal of Agriculture and Biological Sciences, 1(2), 135-141.
- Kobmoo, B., & Hellum, A. K. (1984). Hot water and acid improve the germination of Cassia siamea Britt. seeds. The Embryan, 1(1), 27-33.
- Kumar, D. S., Sharathnath, K. V., Yogeswaran, P., Harani, A., Sudhakar, K., Sudha, P., & Banji, D. (2010). A medicinal potency of Momordica charantia. International Journal of Pharmaceutical Sciences Review and Research, 1(2), 95-100.
- Kumar, G. K., & Thomas, T. D. (2012). High frequency somatic embryogenesis and synthetic seed production in Clitoria ternatea Linn. Plant Cell, Tissue and Organ Culture, 110(1), 141-151. https://doi.org/10.1007/s11240-012-0138-5
- Mog, B., Nayak, J. A. M., & Mohana, G. S. (2017). Germination and seedling establishment in cashew (Anacardium occidentale L.): An interaction between seed size, relative growth rate and seedling biomass. Journal of Plantation Crops, 45(2), 110-120.
- Nguyen, G. K. T., Zhang, S., Nguyen, N. T. K., Nguyen, P. Q. T., Chiu, M. S., Hardjojo, A., & Tam, J. P. (2011). Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of albumin-1 chain a and cyclotide domains in the Fabaceae family. Journal of Biological Chemistry, 286(27), 24275-24287. https://doi.org/10.1074%2Fjbc.M111.229922
- Nimesh, S. (2018). Herbal drug is better than allopathic drug in the treatment of rheumatoid arthritis. International Journal of Pharmacognosy, 5(9), 539-545.
- Nonogaki, H., Bassel, G. W., & Bewley, J. D. (2010). Germination—Still a mystery. Plant Science, 179(6), 574-581. https://doi.org/10.1016/j.plantsci.2010.02.010
- Patwekar, S. L., Suryawanshi, A. B., Gaikwad, M. S., Pedewad, S. R., & Potulwar, A. P. (2016). Standardization of herbal drugs: An overview. The Pharma Innovation Journal, 4(9), 100-104.
- Rajjou, L., Duval, M., Gallardo, K., Catusse, J., Bally, J., Job, C., & Job, D. (2012). Seed germination and vigor. Annual Review of Plant Biology, 63, 507-533. https://doi.org/10.1146/annurev-arplant-042811-105550
- The Seed Site. (2013). Leguminosae. http://theseedsite.co.uk/leguminosae.html]}
