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Pathogenicity of Aeromonas hydrophila in High-value Native Pangasius Catfish, Pangasius nasutus (Bleeker)

Bashir Sani, Md. Sabri Yusoff, Ina Salwany Md Yasin, Murni Marlina Abd Karim, Mohd Fuad Matori, Mohammad Noor Azmai Amal, Tilusha Manchanayake, Amir-Danial Zahaludin and Aslah Mohamad

Pertanika Journal of Tropical Agricultural Science, Volume 46, Issue 2, May 2023

DOI: https://doi.org/10.47836/pjtas.46.2.01

Keywords: Aeromonas hydrophila, LD50, pangasius catfish, Pangasius nasutus, pathogenicity

Published on: 16 May 2023

Pangasius catfish, Pangasius nasutus, is a promising candidate for aquaculture due to its high market value. However, the presence of pathogenic bacteria in Aeromonas hydrophila is a major concern in P. nasutus farming in this country. This study determines the pathogenicity of A. hydrophila in P. nasutus. A total of 80 P. nasutus juveniles were intraperitoneally injected with 0, 103, 105, and 107 CFU mL-1 of A. hydrophila and monitored until 240 hr. The infected moribund fish’s kidneys, livers, and spleens were collected for histopathological analysis. The LD50-240hr value was found at 0.8 × 104 CFU/ml of A. hydrophila. The percentage of mortality in 0, 103, 105, and 107 CFU/ml infected groups were found to be at 0, 40, 60, and 90%, respectively. The infected fish showed congestion at the base of the fin, ascites, enlarged gall bladder, and swollen spleen. It is the earliest report on A. hydrophila’s pathogenicity in high-value native fish, P. nasutus.

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ISSN 1511-3701

e-ISSN 2231-8542

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