Chemical-composition/antioxidant-activity of Satureja hortensis L. extract is influenced by many factors including nutrient elements. A factorial completely randomized design greenhouse experiment was conducted to study the effect of three levels of soil-applied iron, Fe (0, 8 and 16mg kg soil as ethylene-di-amine-die-hydroxyl-acetic-acid, FeEDDHA) and three levels of sulfur, S (0, 50 and 100mg kg^-1 soil as elemental-S) on monoterpene production and antioxidant activity of Satureja hortensis L. The maximum (377.75 mg/L) and minimum (720.406 mg/L) antioxidant activity were obtained with 8mg Fe+100mg S treatment and control, respectively. The main oil constituents in control were γ-terpinene (67%), a-terpinene (11%), myrcene (4%), a-thujene (4%), p-cymene (4%), a-pinene (3%) and carvacrol (2%). The maximum content of a-thujene, a-pinene, myrcene and a-terpinene was obtained with 8mg Fe+50mg S application whereas the control was suitable for obtaining γ-terpinene. Carvacrol was mainly produced with addition of 16mg Fe+100mg S. Furthermore, the a-thujene, a-pinene, myrcene and a-terpinene contents increased with application of 8mg Fe+50mg S.-1 The a-terpinene, myrcene, a-thujene and a-pinene increased by 13, 27, 21 and 43% compared to control, respectively when 8mg Fe+50mg S was applied. The entire component of monoterpenoid fraction with the major constituent of γ-terpinene, a-terpinene, myrcene, a-thujene, p-cymene, a-pinene and carvacrol that constitutes 99.9% of essential oil showed a same trend whereas identified sesquiterpenes and sesquiterpenoid components were relatively low (0.1%). The low molecular weight of γ-terpinene decreased as 8mg Fe+50mg S was applied. In general, it could be concluded that application of 8mg Fe+50mg S kg^-1 the most suitable treatment for obtaining higher amounts of a-terpinene, myrcene, a-thujene and a-pinene whereas addition of 16mg Fe+100mg S kg^-1 was preferable for obtaining carvacrol.