A diversity study of the subfamilies of Braconidae was conducted at Fraser's Hill, Pahang, Malaysia. Sampling took place at three different altitudes using Malaise traps: lower altitude (<500m), intermediate altitude (501-1000m) and higher altitude (>1000m). A total of 572 individuals of braconids were collected from the three altitudes and comprised 15 subfamilies: Agathidinae, Alysiinae, Blacinae, Braconinae, Cheloninae, Doryctinae, Euphorinae, Helconinae, Lysiterminae, Microgastrinae, Miracinae, Opiinae, Orgilinae, Pambolinae and Rogadinae. There were 435 individuals and 55 species, 84 individuals and 30 species, and 53 individuals and 26 species, with a diversity index of H'= 3.75, 2.91 and 3.01, representing the lower, intermediate and higher altitudes, respectively. The diversity index of the lower altitude (H'=3.75) was significantly different from the intermediate (p=0.00, <0.05) and higher altitudes (p=0.00, <0.05). There was no significant difference between the intermediate and higher altitudes (p=0.86, >0.05). This is probably due to the variability of host and food availability i in lowlands compared with highlands.