The effects of crude glycerin, a by-product from palm oil biodiesel production, supplemented in broiler diet on growth performance and carcass quality were studied. Four hundred 1-day-old-male Cobb 500 were randomly allotted into a completely randomized design of five groups consisting of four replications per group. Control diet and diets containing with 2.5%, 5%, 7.5%, and 10% of glycerin in pellet form were fed ad libitum to the broilers from 1 day of age until the age of 42 days. At the end of the experiment, eight broilers per treatment were slaughtered for carcass quality study. From the results, no significant difference was found in live weight change and feed intake with those receiving diets with and without glycerin supplementation. However, low growth performance and carcass yield were indicated when the levels of glycerin increased, particularly at 10%. No effect of dietary treatments on physical properties of the breast meat (P > 0.05). Therefore, this study recommended for mixing crude glycerin not >5% in the broiler diets. Furthermore, the inclusion of glycerin in the diets did not show any influence on the pH, tCIE color, shear force, drip, and cooking loss percentages of the breast meat (P > 0.05).